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Addgene

trLATm1PtoLmEos3.2
(Plasmid #203749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mEos3.2-C1
  • Backbone manufacturer
    Addgene; Michael Davidson's lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    trLATm1 (PtoL)
  • Species
    H. sapiens (human)
  • Mutation
    P8L, P17L
  • Entrez Gene
    LAT (a.k.a. IMD52, LAT1, pp36)
  • Tag / Fusion Protein
    • mEos3.2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Insert DNA courtesy of the Ilya Levental lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    trLATm1PtoLmEos3.2 was a gift from Sarah Veatch (Addgene plasmid # 203749 ; http://n2t.net/addgene:203749 ; RRID:Addgene_203749)

  • For your References section:

    Membrane phase separation drives responsive assembly of receptor signaling domains. Shelby SA, Castello-Serrano I, Wisser KC, Levental I, Veatch SL. Nat Chem Biol. 2023 Jun;19(6):750-758. doi: 10.1038/s41589-023-01268-8. Epub 2023 Mar 30. 10.1038/s41589-023-01268-8 PubMed 36997644
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