mEos3.2trLAT-P
(Plasmid #203747)

• Purpose: Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.
• Depositing Lab: Sarah Veatch
• Publication: Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. doi: 10.1038/s41589-023-01268-8. Epub 2023 Mar 30.

Backbone

• Vector backbone: mEos3.2-C1
• Backbone manufacturer: Addgene; Michael Davidson's lab
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: trLAToC27A
• Species: M. musculus (mouse)
• Mutation: C27A
• Entrez Gene: Lat (a.k.a. p36-38, pp36)
• Tag / Fusion Protein:
  • ER translocation signal - mEos3.2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • mEos3.2trLAT-P.gb
• A portion of this plasmid was derived from a plasmid made by: Insert DNA courtesy of Jonathan Grover in the Akira Ono lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry