pHLP700
(Plasmid #203742)

• Purpose: C-terminal tagging of target proteins with AID*/3xHA degron
• Depositing Lab: Mark Hall
• Publication: Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1128/msphere.00283-23.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: unknown
• Total vector size (bp): 6839
• Vector type: PCR amplification template for gene tagging
• Selectable markers: SAT1 (Nourseothricin)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IAA17 auxin binding domain codons 71-114
• Alt name: AID*
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 282
• Promoter: none
• Tag / Fusion Protein:
  • 3xHA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • pHLP700_wideseq.gbk
• A portion of this plasmid was derived from a plasmid made by: The truncated IAA17 region (called AID*) used as an auxin-dependent degron was originally described here: https://doi.org/10.1002/yea.2967

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry