pHLP712
(Plasmid #203741)

• Purpose: Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus
• Depositing Lab: Mark Hall
• Publication: Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1128/msphere.00283-23.

Backbone

• Vector backbone: unknown
• Total vector size (bp): 9864
• Vector type: Candida albicans integrating vector
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OsTIR1
• Species: Oryza sativa
• Insert Size (bp): 1725
• Mutation: Codon 74 changed from Phe to Ala (F74A)
• Promoter: ACT1 (C. albicans)
• Tag / Fusion Protein:
  • 3xMyc (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

OsTIR1 gene was made synthetically and codon-optimized for Candida albicans