GST TopBP1 D (aa 1-1013)
(Plasmid
#20374)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX6P1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTopBP1 (1-1013)
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Alt nameTOPBP1
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Alt nametopoisomerase II beta binding protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)3039
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Mutationamino acids 1-1013
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Entrez GeneTOPBP1 (a.k.a. Dpb11, TOP2BP1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST TopBP1 D (aa 1-1013) was a gift from Aziz Sancar (Addgene plasmid # 20374 ; http://n2t.net/addgene:20374 ; RRID:Addgene_20374) -
For your References section:
Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Choi JH, Lindsey-Boltz LA, Sancar A. Nucleic Acids Res. 2009 Jan 12. ():. 10.1093/nar/gkn1075 PubMed 19139065
Map uploaded by the depositor.
