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pENTR-nlsABACUS2-400n
(Plasmid #203726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221-f1
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    f1 origin into the NspI site of pDONR221
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nlsABACUS2-400n
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2394
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-nlsABACUS2-400n was a gift from Alexander Jones (Addgene plasmid # 203726 ; http://n2t.net/addgene:203726 ; RRID:Addgene_203726)

  • For your References section:

    Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity. Rowe J, Grange-Guermente M, Exposito-Rodriguez M, Wimalasekera R, Lenz MO, Shetty KN, Cutler SR, Jones AM. Nat Plants. 2023 Jun 26. doi: 10.1038/s41477-023-01447-4. 10.1038/s41477-023-01447-4 PubMed 37365314
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