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Addgene

SuperClomeleon
(Plasmid #203724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerulean linked topaz fluorescent protein
  • Species
    Synthetic
  • Mutation
    Cerulean, modified Topaz Fluorescent Protein with enhanced halide binding affinity, C-terminal Flag, His6 epitope tags

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SuperClomeleon was a gift from George Augustine (Addgene plasmid # 203724 ; http://n2t.net/addgene:203724 ; RRID:Addgene_203724)

  • For your References section:

    Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation. Grimley JS, Li L, Wang W, Wen L, Beese LS, Hellinga HW, Augustine GJ. J Neurosci. 2013 Oct 9;33(41):16297-309. doi: 10.1523/JNEUROSCI.4616-11.2013. 10.1523/JNEUROSCI.4616-11.2013 PubMed 24107961
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