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Addgene

Clomeleon
(Plasmid #203723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyan fluorescent protein linked yellow florescent protein
  • Species
    Synthetic
  • Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Protein, rTEV protease cleavage site, His6 tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Clomeleon was a gift from George Augustine (Addgene plasmid # 203723 ; http://n2t.net/addgene:203723 ; RRID:Addgene_203723)

  • For your References section:

    A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Kuner T, Augustine GJ. Neuron. 2000 Sep;27(3):447-59. doi: 10.1016/s0896-6273(00)00056-8. 10.1016/s0896-6273(00)00056-8 PubMed 11055428
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