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VC-Y705F+K685R-STAT3
(Plasmid #203722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.3 Topo
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5440
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT3 tagged with VC (aa 158-238)
  • Alt name
    APRF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2571
  • Mutation
    Y705F+K685R substitutions
  • Entrez Gene
    STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
  • Promoter CMV
  • Tag / Fusion Protein
    • VC (aa 158-238) for bimolecular fluorescence complementation assays (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VC-Y705F+K685R-STAT3 was a gift from Federico Herrera (Addgene plasmid # 203722 ; http://n2t.net/addgene:203722 ; RRID:Addgene_203722)

  • For your References section:

    Asymmetric post-translational modifications regulate the nuclear translocation of STAT3 homodimers in response to leukemia inhibitory factor. Diallo M, Pimenta C, Murtinheira F, Martins-Alves D, Pinto FR, da Costa AA, Letra-Vilela R, Martin V, Rodriguez C, Rodrigues MS, Herrera F. Cell Oncol (Dordr). 2023 Dec 27. doi: 10.1007/s13402-023-00911-9. 10.1007/s13402-023-00911-9 PubMed 38150153
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