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Addgene

GST TopBP1 B (aa 978-1192)
(Plasmid #20372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TopBP1 (978-1192)
  • Alt name
    TOPBP1
  • Alt name
    topoisomerase II beta binding protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    642
  • Mutation
    amino acids 978-1192
  • Entrez Gene
    TOPBP1 (a.k.a. Dpb11, TOP2BP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST TopBP1 B (aa 978-1192) was a gift from Aziz Sancar (Addgene plasmid # 20372 ; http://n2t.net/addgene:20372 ; RRID:Addgene_20372)

  • For your References section:

    Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Choi JH, Lindsey-Boltz LA, Sancar A. Nucleic Acids Res. 2009 Jan 12. ():. 10.1093/nar/gkn1075 PubMed 19139065
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