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Addgene

pOSML-Cox2-GFP
(Plasmid #203711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOSML
  • Backbone size w/o insert (bp) 5000
  • Modifications to backbone
    pOSML was modified from pMT2 by adding a larger polylinker sequence (PMID:7965814). The GFP tag was inserted at BspEI sist in Cox2 coding sequence. GFP is inserted 18 aa upstream of the C-terminal end of the protein (PMID:10567385).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cox2-GFP
  • Alt name
    PTGS2-GFP
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    PTGS2 (a.k.a. COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2)
  • Promoter SV40
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOSML-Cox2-GFP was a gift from Timothy Hla (Addgene plasmid # 203711 ; http://n2t.net/addgene:203711 ; RRID:Addgene_203711)

  • For your References section:

    Overexpression of cyclooxygenase-2 induces cell cycle arrest. Evidence for a prostaglandin-independent mechanism. Trifan OC, Smith RM, Thompson BD, Hla T. J Biol Chem. 1999 Nov 26;274(48):34141-7. PubMed 10567385
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