pOSML-Cox2-GFP
(Plasmid #203711)

• Purpose: Overexpression of COX2-GFP fusion
• Depositing Lab: Timothy Hla
• Publication: Trifan et al J Biol Chem. 1999 Nov 26;274(48):34141-7.

Backbone

• Vector backbone: pOSML
• Backbone size w/o insert (bp): 5000
• Modifications to backbone: pOSML was modified from pMT2 by adding a larger polylinker sequence (PMID:7965814). The GFP tag was inserted at BspEI sist in Cox2 coding sequence. GFP is inserted 18 aa upstream of the C-terminal end of the protein (PMID:10567385).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cox2-GFP
• Alt name: PTGS2-GFP
• Species: H. sapiens (human)
• Mutation: WT
• Entrez Gene: PTGS2 (a.k.a. COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2)
• Promoter: SV40
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (unknown if destroyed)
• 3′ cloning site: Sal1 (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry