pCDH-Flag-hS1pr1-S5A_Neo
(Plasmid
#203710)
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PurposeOverexpression of FLAG-S1PR1-S5A mutant.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-Neo
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlag-hS1PR1-S5A
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Alt nameS1P1-S5A
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Alt nameEDG1-S5A
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SpeciesH. sapiens (human)
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MutationS5A
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Entrez GeneS1PR1 (a.k.a. CD363, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, S1P1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-Flag-hS1pr1-S5A_Neo was a gift from Timothy Hla (Addgene plasmid # 203710 ; http://n2t.net/addgene:203710 ; RRID:Addgene_203710)