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Addgene

pCDH-Flag-hS1pr1-GFP-Neo
(Plasmid #203708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-Neo
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-hS1PR1-GFP
  • Alt name
    S1P1-GFP
  • Alt name
    EDG1-GFP
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    S1PR1 (a.k.a. CD363, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, S1P1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-Flag-hS1pr1-GFP-Neo was a gift from Timothy Hla (Addgene plasmid # 203708 ; http://n2t.net/addgene:203708 ; RRID:Addgene_203708)
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