pCW-LIC-N8C_Fab2b
(Plasmid
#203692)
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PurposeExpresses N8C_Fab2b
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCW-LIC
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Backbone manufacturerStructural Genomics Consortium
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN8C_Fab2b
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.01.14.524048v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW-LIC-N8C_Fab2b was a gift from Brenda Schulman (Addgene plasmid # 203692 ; http://n2t.net/addgene:203692 ; RRID:Addgene_203692) -
For your References section:
Activity-based profiling of cullin-RING E3 networks by conformation-specific probes. Henneberg LT, Singh J, Duda DM, Baek K, Yanishevski D, Murray PJ, Mann M, Sidhu SS, Schulman BA. Nat Chem Biol. 2023 Dec;19(12):1513-1523. doi: 10.1038/s41589-023-01392-5. Epub 2023 Aug 31. 10.1038/s41589-023-01392-5 PubMed 37653169