pQFT
(Plasmid
#203674)
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Purpose(Empty Backbone) pQF derivative with promoter PQ5 exchanged for promoter PQJ
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQFT
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Backbone manufacturerAndreas Kaczmarczyk
- Backbone size (bp) 7500
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Vector typeBacterial Expression
- Promoter PQJ
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Tags
/ Fusion Proteins
- 3xFLAG (N terminal on backbone)
- 3xFLAG (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 3′ sequencing primer M13 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQFT was a gift from Urs Jenal (Addgene plasmid # 203674 ; http://n2t.net/addgene:203674 ; RRID:Addgene_203674) -
For your References section:
A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene Expression in Pseudomonas aeruginosa. Klotz A, Kaczmarczyk A, Jenal U. Appl Environ Microbiol. 2023 May 18:e0021123. doi: 10.1128/aem.00211-23. 10.1128/aem.00211-23 PubMed 37199671
