pET-16b-10His-TEV-MCES_VACCA_D1_D12
(Plasmid
#203671)
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PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET16b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMCES_VACCA_D1_D12
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Alt nameVaccinia virus capping enzyme
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SpeciesVaccinia virus
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Tag
/ Fusion Protein
- 10xHis (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-16b-10His-TEV-MCES_VACCA_D1_D12 was a gift from Stephen Cusack (Addgene plasmid # 203671 ; http://n2t.net/addgene:203671 ; RRID:Addgene_203671) -
For your References section:
Crystal structure of vaccinia virus mRNA capping enzyme provides insights into the mechanism and evolution of the capping apparatus. Kyrieleis OJ, Chang J, de la Pena M, Shuman S, Cusack S. Structure. 2014 Mar 4;22(3):452-65. doi: 10.1016/j.str.2013.12.014. 10.1016/j.str.2013.12.014 PubMed 24607143
