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Addgene

PB-NLS-ZapCV2-ALA
(Plasmid #203665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyback
  • Backbone manufacturer
    Liu Laboratory at CU Boulder
  • Backbone size w/o insert (bp) 6929
  • Total vector size (bp) 8505
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZapCV2-Ala zinc dead sensor
  • Alt name
    NLS-ZapCV2-Ala
  • Species
    Synthetic
  • Insert Size (bp)
    1576
  • Mutation
    Cys and His in Zap1 is mutated with Ala
  • GenBank ID
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BanHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-NLS-ZapCV2-ALA was a gift from Amy Palmer (Addgene plasmid # 203665 ; http://n2t.net/addgene:203665 ; RRID:Addgene_203665)

  • For your References section:

    Human cells experience a Zn(2+) pulse in early G1. Rakshit A, Holtzen SE, Lo MN, Conway KA, Palmer AE. Cell Rep. 2023 Jun 16;42(6):112656. doi: 10.1016/j.celrep.2023.112656. 10.1016/j.celrep.2023.112656 PubMed 37330912
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