BSD-P2A-TagRFP-hSmad1-donor
(Plasmid
#203662)
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PurposeDonor plasmid for CRISPR knock-in
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBSD-P2A-tagRFP
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesmad1
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SpeciesH. sapiens (human)
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Entrez GeneSMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
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Tag
/ Fusion Protein
- RFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BSD-P2A-TagRFP-hSmad1-donor was a gift from Ali Hemmati Brivanlou (Addgene plasmid # 203662 ; http://n2t.net/addgene:203662 ; RRID:Addgene_203662) -
For your References section:
WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids. Yoney A, Etoc F, Ruzo A, Carroll T, Metzger JJ, Martyn I, Li S, Kirst C, Siggia ED, Brivanlou AH. Elife. 2018 Oct 12;7:e38279. doi: 10.7554/eLife.38279. 10.7554/eLife.38279 PubMed 30311909
Map uploaded by the depositor.
