pINO4-NLS-Pfv-Sapphire-HO
(Plasmid
#203660)
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PurposeYeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSIB843
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePfV
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SpeciesPyrococcus furiosus
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Tag
/ Fusion Protein
- Sapphire (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.18.469159 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINO4-NLS-Pfv-Sapphire-HO was a gift from Liam Holt (Addgene plasmid # 203660 ; http://n2t.net/addgene:203660 ; RRID:Addgene_203660) -
For your References section:
nucGEMs probe the biophysical properties of the nucleoplasm. Shu T et al.. bioRxiv 2022 10.1101/2021.11.18.469159