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Addgene

pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
(Plasmid #203655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203655 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PfV
  • Species
    Pyrococcus furiosus
  • Tag / Fusion Protein
    • Sapphire (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.18.469159 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire was a gift from Liam Holt (Addgene plasmid # 203655 ; http://n2t.net/addgene:203655 ; RRID:Addgene_203655)

  • For your References section:

    nucGEMs probe the biophysical properties of the nucleoplasm. Shu T et al.. bioRxiv 2022 10.1101/2021.11.18.469159
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