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Addgene

pCDH1-CMV-ATG3-C264A-mCherry-Hygro
(Plasmid #203638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203638 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDH1-CMV-MCS-SV40-Hygro
  • Vector type
    Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATG3
  • Alt name
    ATG3 (1-125)-mCherry-ATG3 (126-314)-C264A-stop
  • Species
    H. sapiens (human)
  • Mutation
    C264A
  • Entrez Gene
    ATG3 (a.k.a. APG3, APG3-LIKE, APG3L, PC3-96, hApg3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH1-CMV-ATG3-C264A-mCherry-Hygro was a gift from James Hurley (Addgene plasmid # 203638)
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