FB395
(Plasmid
#203631)
-
PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 203631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDGB3alpha2
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameR1:G1aG2b.1:mPAF:Luc:TtrpC
-
SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FB395 was a gift from Jose Marcos (Addgene plasmid # 203631 ; http://n2t.net/addgene:203631 ; RRID:Addgene_203631) -
For your References section:
FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi. Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez D, Marcos JF, Garrigues S. Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 10.3389/fbioe.2023.1222812. eCollection 2023. 10.3389/fbioe.2023.1222812 PubMed 37609115