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pNKX2-5-T2A-GFP-PGK-Puro
(Plasmid #203607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 3522
  • Total vector size (bp) 6301
  • Vector type
    Human Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NKX2-5-T2A-GFP-PGK-Puro-NKX2-5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3522
  • GenBank ID
  • Entrez Gene
    NKX2-5 (a.k.a. CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer GTCATAGCTGTTTCCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNKX2-5-T2A-GFP-PGK-Puro was a gift from Matthew Birket (Addgene plasmid # 203607 ; http://n2t.net/addgene:203607 ; RRID:Addgene_203607)

  • For your References section:

    The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2. Douglas M, O'Loughlin C, Lynch AT, Prialgauskaite R, Adamson AD, Dibb KM, Kimber SJ, Birket MJ. Stem Cell Res. 2023 Dec 3;74:103262. doi: 10.1016/j.scr.2023.103262. 10.1016/j.scr.2023.103262 PubMed 38100908
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