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Addgene

CSII-TK-MAIP1
(Plasmid #203599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CSII-TK-MCS
  • Backbone size w/o insert (bp) 5339
  • Modifications to backbone
    9000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    matrix AAA peptidase interacting protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    774
  • Mutation
    WT
  • GenBank ID
    NM_001369399.1
  • Entrez Gene
    MAIP1 (a.k.a. C2orf47)
  • Promoter TK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CGGGTCATAAAAATGGCGCTGG
  • 3′ sequencing primer AAGTCCATAAGCTGATTTTTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CSII-TK-MAIP1 was a gift from Hiroyuki Mizuguchi (Addgene plasmid # 203599 ; http://n2t.net/addgene:203599 ; RRID:Addgene_203599)

  • For your References section:

    miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, Sakurai F, Mizuguchi H. Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w. 10.1038/s42003-023-05049-w PubMed 37355744
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