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CSII-TK-B4GALT3
(Plasmid #203598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CSII-TK-MCS
  • Backbone size w/o insert (bp) 5339
  • Total vector size (bp) 9610
  • Modifications to backbone
    ORF of B4GLAT3 was cloned between NotI and XbaI in CSII-TK-MCS
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta-1,4-galactosyltransferase 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1384
  • Mutation
    WT
  • GenBank ID
    NM_001199873.1
  • Entrez Gene
    B4GALT3 (a.k.a. beta4Gal-T3)
  • Promoter TK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GCCGCCGGTTTCATGATTAAGGTAGAC
  • 3′ sequencing primer GTGCTGGATGGAGCCTTTCTCTGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CSII-TK-B4GALT3 was a gift from Hiroyuki Mizuguchi (Addgene plasmid # 203598 ; http://n2t.net/addgene:203598 ; RRID:Addgene_203598)

  • For your References section:

    miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, Sakurai F, Mizuguchi H. Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w. 10.1038/s42003-023-05049-w PubMed 37355744
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