CSII-TK-MCS
(Plasmid
#203597)
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Purpose(Empty Backbone) Empty lentiviral vector to express protein in mammalian cells cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCSII-EF-MCS
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Backbone manufacturerRIKEN-BRC
- Backbone size (bp) 9136
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Modifications to backboneEF1a promoter was replaced with TK promoter
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Vector typeMammalian Expression, Lentiviral
- Promoter TK
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CSII-TK-MCS was a gift from Hiroyuki Mizuguchi (Addgene plasmid # 203597 ; http://n2t.net/addgene:203597 ; RRID:Addgene_203597) -
For your References section:
miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, Sakurai F, Mizuguchi H. Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w. 10.1038/s42003-023-05049-w PubMed 37355744