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pPSt_35Spr-GFP-t35S
(Plasmid #203592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPSt
  • Backbone manufacturer
    Jores et al, 2023
  • Backbone size w/o insert (bp) 4534
  • Total vector size (bp) 5672
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CaMV 35S promoter + Zm00001d041672 5'UTR
  • Species
    Cauliflower mosaic virus + Zea mays
  • Insert Size (bp)
    293

Gene/Insert 2

  • Gene/Insert name
    eGFP
  • Alt name
    GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    717

Gene/Insert 3

  • Gene/Insert name
    CaMV 35S terminator
  • Alt name
    t35S
  • Species
    Cauliflower mosaic virus
  • Insert Size (bp)
    204

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPSt_35Spr-GFP-t35S was a gift from Stan Fields (Addgene plasmid # 203592 ; http://n2t.net/addgene:203592 ; RRID:Addgene_203592)

  • For your References section:

    Arabidopsis and maize terminator strength is determined by GC content, polyadenylation motifs and cleavage probability. Gorjifard S, Jores T, Tonnies J, Mueth NA, Bubb K, Wrightsman T, Buckler ES, Fields S, Cuperus JT, Queitsch C. Nat Commun. 2024 Jul 12;15(1):5868. doi: 10.1038/s41467-024-50174-7. 10.1038/s41467-024-50174-7 PubMed 38997252
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