pACRISPR-KM
(Plasmid
#203587)
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Purpose(Empty Backbone) A sgRNA expression plasmid for genome editing in Burkholderia
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACRISPR
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Backbone manufacturerThis was built on the plasmid pACRISPR (addgene reference:#113348) from Quanjiang Ji
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACRISPR-KM was a gift from Leonilde Moreira (Addgene plasmid # 203587 ; http://n2t.net/addgene:203587 ; RRID:Addgene_203587) -
For your References section:
Genomic editing in Burkholderia multivorans by CRISPR/Cas9. Ferreira MR, Queiroga V, Moreira LM. Appl Environ Microbiol. 2024 Feb 21;90(2):e0225023. doi: 10.1128/aem.02250-23. Epub 2024 Feb 2. 10.1128/aem.02250-23 PubMed 38299816
