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pLX304-EZH1-Y727F
(Plasmid #203582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Backbone size w/o insert (bp) 9377
  • Total vector size (bp) 9963
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EZH1
  • Alt name
    Enhancer of zest 1 Polycomb repressive complex 2
  • Alt name
    KMT6B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2313
  • Mutation
    changed Tyrosine 727 to Phenylalanine for partial resistance to JQ-EZ-05
  • GenBank ID
    2145
  • Entrez Gene
    EZH1 (a.k.a. KMT6B)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5-tagged (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Broad TRC (ccsbBroad304_00525)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations encoding the Y727F were introduced by site-directed mutagenesis into the pLX304-EZH1 backbone

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-EZH1-Y727F was a gift from Abhishek Chakraborty & William Kaelin (Addgene plasmid # 203582 ; http://n2t.net/addgene:203582 ; RRID:Addgene_203582)

  • For your References section:

    HIF activation causes synthetic lethality between the VHL tumor suppressor and the EZH1 histone methyltransferase. Chakraborty AA, Nakamura E, Qi J, Creech A, Jaffe JD, Paulk J, Novak JS, Nagulapalli K, McBrayer SK, Cowley GS, Pineda J, Song J, Wang YE, Carr SA, Root DE, Signoretti S, Bradner JE, Kaelin WG Jr. Sci Transl Med. 2017 Jul 12;9(398):eaal5272. doi: 10.1126/scitranslmed.aal5272. 10.1126/scitranslmed.aal5272 PubMed 28701475
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