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pLX304-ARNT-d414
(Plasmid #203579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Backbone size w/o insert (bp) 9377
  • Total vector size (bp) 10089
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARNT
  • Alt name
    ARNT1
  • Alt name
    HIF1B
  • Alt name
    aryl hydrocarbon receptor nuclear translator
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2439
  • Mutation
    5' 414 basepair deletion [Δ414]
  • GenBank ID
    405
  • Entrez Gene
    ARNT (a.k.a. ARNT1, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-terminal deletion of the DNA-binding domain was generated using an internal forward primer. D414 ARNT was sub-cloned using gateway cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-ARNT-d414 was a gift from Abhishek Chakraborty & William Kaelin (Addgene plasmid # 203579 ; http://n2t.net/addgene:203579 ; RRID:Addgene_203579)

  • For your References section:

    Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Chakraborty AA, Laukka T, Myllykoski M, Ringel AE, Booker MA, Tolstorukov MY, Meng YJ, Meier SR, Jennings RB, Creech AL, Herbert ZT, McBrayer SK, Olenchock BA, Jaffe JD, Haigis MC, Beroukhim R, Signoretti S, Koivunen P, Kaelin WG Jr. Science. 2019 Mar 15;363(6432):1217-1222. doi: 10.1126/science.aaw1026. Epub 2019 Mar 14. 10.1126/science.aaw1026 PubMed 30872525
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