pLX304-ARNT
(Plasmid
#203578)
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PurposeExpresses wild-type ARNT in mammalian cells.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX304
- Backbone size w/o insert (bp) 9377
- Total vector size (bp) 10089
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARNT
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Alt nameARNT1
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Alt nameHIF1B
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Alt namearyl hydrocarbon receptor nuclear translator
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SpeciesH. sapiens (human)
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Insert Size (bp)2439
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GenBank ID405
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Entrez GeneARNT (a.k.a. ARNT1, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Wild-type ARNT was sub-cloned using gateway cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX304-ARNT was a gift from Abhishek Chakraborty & William Kaelin (Addgene plasmid # 203578 ; http://n2t.net/addgene:203578 ; RRID:Addgene_203578) -
For your References section:
Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Chakraborty AA, Laukka T, Myllykoski M, Ringel AE, Booker MA, Tolstorukov MY, Meng YJ, Meier SR, Jennings RB, Creech AL, Herbert ZT, McBrayer SK, Olenchock BA, Jaffe JD, Haigis MC, Beroukhim R, Signoretti S, Koivunen P, Kaelin WG Jr. Science. 2019 Mar 15;363(6432):1217-1222. doi: 10.1126/science.aaw1026. Epub 2019 Mar 14. 10.1126/science.aaw1026 PubMed 30872525