pH7m34GW-35S:SNACS
(Plasmid
#203576)
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PurposePlant SnRK2 kinase activity FRET sensor in plant expression vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203576 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepH7m34GW
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNACS
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SpeciesSynthetic
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Mutationnone
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Tag
/ Fusion Protein
- StrepII (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH7m34GW-35S:SNACS was a gift from Julian Schroeder (Addgene plasmid # 203576 ; http://n2t.net/addgene:203576 ; RRID:Addgene_203576) -
For your References section:
FRET kinase sensor development reveals SnRK2/OST1 activation by ABA but not by MeJA and high CO(2) during stomatal closure. Zhang L, Takahashi Y, Hsu PK, Kollist H, Merilo E, Krysan PJ, Schroeder JI. Elife. 2020 May 28;9:e56351. doi: 10.7554/eLife.56351. 10.7554/eLife.56351 PubMed 32463362
Map uploaded by the depositor.
