FB323
(Plasmid
#203570)
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PurposeModule for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDGB3α1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FB323 was a gift from Jose Marcos (Addgene plasmid # 203570 ; http://n2t.net/addgene:203570 ; RRID:Addgene_203570) -
For your References section:
Development of a FungalBraid Penicillium expansum-based expression system for the production of antifungal proteins in fungal biofactories. Gandia M, Moreno-Gimenez E, Giner-Llorca M, Garrigues S, Ropero-Perez C, Locascio A, Martinez-Culebras PV, Marcos JF, Manzanares P. Microb Biotechnol. 2022 Feb;15(2):630-647. doi: 10.1111/1751-7915.14006. Epub 2022 Jan 27. 10.1111/1751-7915.14006 PubMed 35084102