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Addgene

pCAG-VPS15-TSF
(Plasmid #203563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS15
  • Species
    H. sapiens (human)
  • GenBank ID
    AAI27106.1
  • Entrez Gene
    PIK3R4 (a.k.a. VPS15, p150)
  • Promoter CMV
  • Tag / Fusion Protein
    • TwinStrep Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

VPS15-TEVsite-TwinStrep-Flag-stop

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-VPS15-TSF was a gift from James Hurley (Addgene plasmid # 203563 ; http://n2t.net/addgene:203563 ; RRID:Addgene_203563)

  • For your References section:

    Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH. bioRxiv 2023.06.01.543278 10.1101/2023.06.01.543278
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