pCAG-ULK1 (836-1050)-MBP
(Plasmid
#203562)
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PurposeMammalian expression of ULK1 (836-1050) with MBP tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameULK1
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SpeciesH. sapiens (human)
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GenBank IDNP_003556.2
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Entrez GeneULK1 (a.k.a. ATG1, ATG1A, UNC51, Unc51.1, hATG1)
- Promoter CMV
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Tag
/ Fusion Protein
- MBP-10His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ULK1 (836-1050)-TEVsite-MBP-10His-stop
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-ULK1 (836-1050)-MBP was a gift from James Hurley (Addgene plasmid # 203562 ; http://n2t.net/addgene:203562 ; RRID:Addgene_203562) -
For your References section:
Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH. bioRxiv 2023.06.01.543278 10.1101/2023.06.01.543278