pCDNA3 Flag p38 beta2 (agf)
(Plasmid #20356)

• Depositing Lab: Roger Davis
• Publication: Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pDCNA3 Flag p38 beta(agf)
• Alt name: MapK11
• Species: H. sapiens (human)
• Insert Size (bp): 1212
• Mutation: Replace Thr 182 and Tyr 184 with Ala and Phe respectively
• GenBank ID: AF031135
• Entrez Gene: MAPK11 (a.k.a. P38B, P38BETA2, PRKM11, SAPK2, SAPK2B, p38-2, p38Beta)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xho 1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are two additional mutations in the sequence - Q22L and M198L. These mutations are correct and the sequence still functions normally.