pCAG-TSF-ATG13 (363-517)
(Plasmid
#203550)
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PurposeMammalian expression of TwinStrep-Flag tagged ATG13 (363-517)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG13
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SpeciesH. sapiens (human)
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GenBank IDNP_001333259.1
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Entrez GeneATG13 (a.k.a. KIAA0652, PARATARG8)
- Promoter CMV
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Tag
/ Fusion Protein
- TwinStrep Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XmaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TwinStrep-Flag-TEVsite-ATG13 (363-517)-stop
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-TSF-ATG13 (363-517) was a gift from James Hurley (Addgene plasmid # 203550 ; http://n2t.net/addgene:203550 ; RRID:Addgene_203550) -
For your References section:
Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH. bioRxiv 2023.06.01.543278 10.1101/2023.06.01.543278