pWS-TK3
(Plasmid #20349)

• Purpose: (Empty Backbone)
• Depositing Lab: Mario Capecchi
• Publication: Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Backbone

• Vector backbone: N/A
• Backbone size (bp): 6379
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pWS-TK3
• Species: cloning vector
• GenBank ID: EU530624

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.