pDEST17-HO1(51-231)
(Plasmid
#203486)
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PurposeFor recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST17
- Total vector size (bp) 5432
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Vector typeBacterial Expression, Plant Expression
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Selectable markersno maker
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)699
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-HO1(51-231) was a gift from Yutaka Kodama (Addgene plasmid # 203486 ; http://n2t.net/addgene:203486 ; RRID:Addgene_203486) -
For your References section:
Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status. Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y. Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. Epub 2023 Jun 7. 10.1126/sciadv.adh4787 PubMed 37285441