pGWB511-TP-BVRA
(Plasmid
#203480)
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PurposeFor Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB511
- Total vector size (bp) 11554
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Vector typeBacterial Expression, Plant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
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SpeciesR. norvegicus (rat)
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Insert Size (bp)912
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB511-TP-BVRA was a gift from Yutaka Kodama (Addgene plasmid # 203480 ; http://n2t.net/addgene:203480 ; RRID:Addgene_203480) -
For your References section:
Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status. Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y. Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. Epub 2023 Jun 7. 10.1126/sciadv.adh4787 PubMed 37285441