pWR89
(Plasmid
#203423)
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Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWR88
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Backbone manufacturerRobertson
- Backbone size w/o insert (bp) 8072
- Total vector size (bp) 10189
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Modifications to backboneA section of S. stipitis genomic DNA around the EGC3 gene was added to pWR88 to serve as a chromosomal integration site.
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Vector typeYeast Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGC3 plus flanking
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SpeciesS. stipitis (yeast)
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Insert Size (bp)2111
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Entrez GeneEGC3 (a.k.a. PICST_39320)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer ATCTTAAGGTGGCCAACTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bycoCBG is a codon optimized version of Promega's CBG99 which was synthesized by GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWR89 was a gift from J. Brian Robertson (Addgene plasmid # 203423 ; http://n2t.net/addgene:203423 ; RRID:Addgene_203423) -
For your References section:
BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset for repeatedly modifying wild-type Scheffersomyces stipitis. Reichard WD, Smith SE, Robertson JB. mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. Epub 2023 Jun 22. 10.1128/msphere.00224-23 PubMed 37345937