pRSETa mEos2
(Plasmid #20341)

• Purpose: bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)
• Depositing Lab: Loren Looger
• Publication: McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Backbone

• Vector backbone: pRSETa
• Backbone size w/o insert (bp): 2897
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mEos-2
• Species: E coli
• Insert Size (bp): 681
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry