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Addgene

pET28Man
(Plasmid #203399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28A(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    man2A
  • Species
    Cellulomonas fimi
  • Insert Size (bp)
    2529
  • Mutation
    None
  • GenBank ID
    AF126472
  • Promoter T7
  • Tag / Fusion Protein
    • H6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer 5 prime
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATCC 484

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28Man was a gift from David Vocadlo (Addgene plasmid # 203399 ; http://n2t.net/addgene:203399 ; RRID:Addgene_203399)

  • For your References section:

    Synthesis of 4-methylumbelliferyl alpha-d-mannopyranosyl-(1-->6)-beta-d-mannopyranoside and development of a coupled fluorescent assay for GH125 exo-alpha-1,6-mannosidases. Deng L, Tsybina P, Gregg KJ, Mosi R, Zandberg WF, Boraston AB, Vocadlo DJ. Bioorg Med Chem. 2013 Aug 15;21(16):4839-45. doi: 10.1016/j.bmc.2013.05.062. Epub 2013 Jun 11. 10.1016/j.bmc.2013.05.062 PubMed 23816041
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