pRsetB-his7-Perceval
(Plasmid #20336)

• Depositing Lab: Gary Yellen
• Publication: Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

Backbone

• Vector backbone: pRset B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2764
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Perceval (fluorescent ATP:ADP sensor)
• Species: Synthetic
• Insert Size (bp): 1778
• Tag / Fusion Protein:
  • his(7) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 reverse primer

Resource Information

• Supplemental Documents:
  • Perceval
• A portion of this plasmid was derived from a plasmid made by: synthetic gene
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry