pSB-BbsI-sgRNA
(Plasmid
#203359)
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PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSleeping Beauty
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBbsI Cut Sites
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gRNA/shRNA sequenceVariable
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB-BbsI-sgRNA was a gift from Jonathan Brunger (Addgene plasmid # 203359 ; http://n2t.net/addgene:203359 ; RRID:Addgene_203359) -
For your References section:
Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells. Gil M, Hamann CA, Brunger JM, Gama V. Bio Protoc. 2024 Jan 5;14(1):e4913. doi: 10.21769/BioProtoc.4913. eCollection 2024 Jan 5. 10.21769/BioProtoc.4913 PubMed 38213321
Map uploaded by the depositor.
