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Addgene

pSB-TRE-CRISPRoff-EF1A-TetOn
(Plasmid #203355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Sleeping Beauty
  • Total vector size (bp) 13246
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRISPRoff-TetOn
  • Alt name
    Dnmt3-dCas9-HA-NLS-NLS-TagBFP-KRAB

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB-TRE-CRISPRoff-EF1A-TetOn was a gift from Jonathan Brunger (Addgene plasmid # 203355 ; http://n2t.net/addgene:203355 ; RRID:Addgene_203355)

  • For your References section:

    Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells. Gil M, Hamann CA, Brunger JM, Gama V. Bio Protoc. 2024 Jan 5;14(1):e4913. doi: 10.21769/BioProtoc.4913. eCollection 2024 Jan 5. 10.21769/BioProtoc.4913 PubMed 38213321
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