pMALcPP-MS2cp
(Plasmid #203351)

• Purpose: Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria
• Depositing Lab: Thomas Cech
• Publication: Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;120(26):e2220537120. doi: 10.1073/pnas.2220537120. Epub 2023 Jun 20.

Backbone

• Vector backbone: pMAL-c6T
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 5219
• Total vector size (bp): 5609
• Modifications to backbone: TEV protease recognition site replaced with rhinovirus 3C protease (PreScission Protease) recognition site.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MS2 coat protein
• Alt name: MS2-CP
• Species: Escherichia phage MS2
• Insert Size (bp): 390
• Mutation: V75E and A81G, to prevent capsid assembly
• GenBank ID: NC_001417
• Tag / Fusion Protein:
  • Hx6-MBP (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lab of Roy Parker, University of Colorado Boulder

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.30.518605 for bioRxiv preprint.