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pMALcPP-MS2cp
(Plasmid #203351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203351 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c6T
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 5219
  • Total vector size (bp) 5609
  • Modifications to backbone
    TEV protease recognition site replaced with rhinovirus 3C protease (PreScission Protease) recognition site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MS2 coat protein
  • Alt name
    MS2-CP
  • Species
    Escherichia phage MS2
  • Insert Size (bp)
    390
  • Mutation
    V75E and A81G, to prevent capsid assembly
  • GenBank ID
    NC_001417
  • Tag / Fusion Protein
    • Hx6-MBP (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Lab of Roy Parker, University of Colorado Boulder

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.30.518605 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMALcPP-MS2cp was a gift from Thomas Cech (Addgene plasmid # 203351 ; http://n2t.net/addgene:203351 ; RRID:Addgene_203351)

  • For your References section:

    Multiple RNA- and DNA-binding proteins exhibit direct transfer of polynucleotides with implications for target-site search. Hemphill WO, Voong CK, Fenske R, Goodrich JA, Cech TR. Proc Natl Acad Sci U S A. 2023 Jun 27;120(26):e2220537120. doi: 10.1073/pnas.2220537120. Epub 2023 Jun 20. 10.1073/pnas.2220537120 PubMed 37339225
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