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pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
(Plasmid #203350)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM4Z
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2820
  • Total vector size (bp) 3336
  • Vector type
    Template for T7-mediated in vitro transcription of mRNA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nano Luciferase
  • Alt name
    NanoLuc
  • Alt name
    NLuc
  • Species
    Oplophorus gracilis
  • Insert Size (bp)
    516
  • Promoter T7
  • Tag / Fusion Protein
    • Flanked by 5' and 3' UTRs from Xenopus beta globin and a 64nt polyA tail

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer AATACGACTCACTATAGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Template should be linearized with SpeI for IVT to give 834nt transcript
The construct was found to have a 74 bp polyA sequence rather than 64 bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64 was a gift from Christopher Grigsby & Molly Stevens (Addgene plasmid # 203350 ; http://n2t.net/addgene:203350 ; RRID:Addgene_203350)
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