-
PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
-
Depositing Labs
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGEM4Z
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2820
- Total vector size (bp) 3540
-
Vector typeTemplate for T7-mediated in vitro transcription of mRNA
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEnhanced Green Fluorescent Protein
-
Alt nameEGFP
-
SpeciesAequorea victoria
-
Insert Size (bp)720
- Promoter T7
-
Tag
/ Fusion Protein
- Flanked by 5' and 3' UTRs from Xenopus beta globin and a 64nt polyA tail
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer AATACGACTCACTATAGG (Common Sequencing Primers)
Resource Information
-
Addgene Notes
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Template should be linearized with SpeI for IVT to give 1038nt transcript
The construct was found to have a 74 bp polyA sequence rather than 64 bp
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64 was a gift from Christopher Grigsby & Molly Stevens (Addgene plasmid # 203348 ; http://n2t.net/addgene:203348 ; RRID:Addgene_203348)
