pML523
(Plasmid
#203345)
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Purpose(Empty Backbone) Plasmid backbone for choline sensing circuits. The plasmid contains, lacI and tetR as well as betIM. The plasmid also contains the p15a ori
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepML523
- Backbone size (bp) 5305
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pML523 was a gift from Lauren Andrews (Addgene plasmid # 203345 ; http://n2t.net/addgene:203345 ; RRID:Addgene_203345) -
For your References section:
Algorithmic Programming of Sequential Logic and Genetic Circuits for Recording Biochemical Concentration in a Probiotic Bacterium. Lebovich M, Zeng M, Andrews LB. ACS Synth Biol. 2023 Aug 15. doi: 10.1021/acssynbio.3c00232. 10.1021/acssynbio.3c00232 PubMed 37581922